單克隆抗狂犬病

單克隆抗體抗A抗B原料（狂犬病）

Monoclonal Anti-Rabies, FITC

用直接免疫熒光技術(shù)檢測狂犬病毒。單克隆抗狂犬病，F(xiàn)ITC用于印象涂片檢測（準(zhǔn)備，例如，從可疑的動物腦組織）或用直接免疫熒光技術(shù)在細(xì)胞培養(yǎng)分離病毒。傳統(tǒng)的狂犬病病毒敏感的檢測以及蝙蝠相關(guān)的狂犬病病毒屬，尤其是和EBLV-2 EBLV-1

Monoclonal Anti-Rabies, FITC

Detection of rabies virus using the direct immunfluores-cence technique. Monoclonal Anti-Rabies, FITC is used for detection in impression smears (prepared, for example, from brain tissues of suspicious animals) or by virus isolation in cell cultures using the direct immunofluores- cence technique. Sensitive detection of the traditional rabies virus as well as bat-associated Lyssaviruses, especially EBLV-1 and EBLV-2

單克隆抗體抗A抗B原料（狂犬病）

單克隆抗體(MAb)與抗血清(又稱多克隆抗體，PAb)zui主要的區(qū)別是MAb為單一種B細(xì)胞克隆所產(chǎn)生的一種均一的免疫球蛋白分子。所以MAb是B細(xì)胞克隆的標(biāo)志，是一種*型的抗體，它的特異性是針對一個抗原決定簇的。制備單克隆抗體不能用化學(xué)分離的方法從多克隆抗體中去分離純化得到它，而是用分離產(chǎn)生抗體的B細(xì)胞克隆的方法得到它。為了使B細(xì)胞克隆能在體外人工培養(yǎng)下長期存活并產(chǎn)生*均一的MAb，G．KÖhler合Milstein于1975年創(chuàng)立了雜交瘤方法。所以制備單克隆抗體的技術(shù)又稱雜交瘤技術(shù)(hybredoma technique)。

雜交瘤技術(shù)的基本原理是用分泌抗體但不能長期培養(yǎng)的B細(xì)胞與能在體外長期培養(yǎng)并可低溫保存的腫瘤細(xì)胞進(jìn)行雜交。篩選得到的雜交瘤細(xì)胞應(yīng)該是既能分泌抗體又有瘤細(xì)胞的特性，可長期傳代培養(yǎng)，又可在液氮中保存的細(xì)胞。把這些細(xì)胞單克隆化，用單克隆化的雜交瘤細(xì)胞進(jìn)行單克隆抗體的生產(chǎn)。

The basic principle of hybridoma technique is to hybridize B cells that secrete antibodies but can not be cultured for a long time with cancer cells that can be cultured in vitro and can be preserved at low temperature in vitro. The screened hybridoma cells should be capable of secreting antibodies and tumor cells, which can be cultured for a long time and can be stored in liquid nitrogen. These cells were mcabled and monoclonal antibodies were produced with monoclonal hybridoma cells.

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【公司名稱】 廣州健侖生物科技有限公司
【】    楊永漢

【】 
【騰訊 】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

抗體是在對抗原刺激的免疫應(yīng)答中，B淋巴細(xì)胞產(chǎn)生的一類糖蛋白。它是能與相應(yīng)抗原特異的結(jié)合、產(chǎn)生各種免疫效應(yīng)（生理效應(yīng)）的球蛋白。衛(wèi)生組織將具有抗體活性及化學(xué)結(jié)構(gòu)與抗體相似的一類蛋白統(tǒng)一命名為免疫球蛋白，它與抗體都是指同一類蛋白質(zhì)。70000之間，稱為“重鏈”（H鏈）。
免疫球蛋白結(jié)構(gòu)
免疫球蛋白結(jié)構(gòu)
輕、重鏈之間和兩重鏈之間由二硫橋連接，這樣的四鏈結(jié)構(gòu)（L2H2）組成一個免疫球蛋白分子。以重鏈抗原性差異來進(jìn)行抗體的血清學(xué)分類：用分離純化的各種重鏈免疫動物獲得相應(yīng)的抗血清，再通過免疫交叉反應(yīng)等血清學(xué)檢測方法，分析其結(jié)構(gòu)的異同，反復(fù)的驗(yàn)證，zui后發(fā)現(xiàn)人類有5類不同的重鏈，分別為）與多種細(xì)胞結(jié)合，可能造成免疫損傷。IgG占成人血清中抗體總量的75%以上，是人類血清中主要的一類抗體，由B細(xì)胞產(chǎn)生，其功能結(jié)構(gòu)也是研究zui清楚的。它主要的生理功能有：(1)中和毒素和病毒；(2)凝集和沉淀抗原；(3)激活補(bǔ)體；(4)通過特異的膜受體（Fc單克隆抗體技術(shù)的應(yīng)用是現(xiàn)代生物科學(xué)領(lǐng)域中的重要進(jìn)展之一。

The most important difference between the monoclonal antibody (MAb) and the antiserum (also called the polyclonal antibody, PAb) is that MAb is a homogeneous immunoglobulin produced by a single B cell clone. Therefore, MAb is a marker of B cell clone, and is a unique type of antibody. Its specificity is directed against an antigenic determinant. The preparation of monoclonal antibodies can not be separated and purified from polyclonal antibody by chemical separation, but it is obtained by the method of cloning the B cell that separates the antibody. In order to make B cell clones capable of long-term survival in vitro and produce a fully homogeneous MAb, G.KÖ hler and Milstein established a hybridoma method in 1975. Therefore, the preparation of monoclonal antibodies is also known as hybridoma technology (hybredoma technique).