核酸試劑 艱難梭菌多重檢測(cè)試劑盒（PCR-熒光探針法）

廣州健侖生物科技有限公司

單管多重檢測(cè)艱難梭菌及內(nèi)部對(duì)照。
One tube multiplex for detection of Clostridium difficile and internal control.

核酸試劑 艱難梭菌多重檢測(cè)試劑盒（PCR-熒光探針法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

解決陰性染色的問題非常簡(jiǎn)單，就是設(shè)立“陽(yáng)性對(duì)照”。如果陽(yáng)性對(duì)照有了表達(dá)，說(shuō)明染色的全過程和所有試劑都沒有問題。如果此時(shí)測(cè)試片仍為陰性，便是真實(shí)的陰性，說(shuō)明組織或細(xì)胞沒有相應(yīng)的抗原表達(dá)。反之，如果陽(yáng)性對(duì)照沒有著色，表明染色過程中某個(gè)或某些步驟出了問題或試劑出了問題。應(yīng)一一尋找原因。陽(yáng)性對(duì)照包括兩種，一種稱為“自身對(duì)照”或“內(nèi)部對(duì)照”，這是指在測(cè)試的切片中本身就存在已知的抗原，如正常淋巴結(jié)中存在T和B細(xì)胞抗原，CD20或CD3都應(yīng)該有表達(dá)。自身對(duì)照是一種比較理想的對(duì)照，對(duì)照和測(cè)試組織或細(xì)胞都在同一張切片中，都處于相同的試驗(yàn)條件下，結(jié)果更可靠也更具有可比性。在選擇自身對(duì)照片時(shí)選擇既有病變組織同時(shí)又有正常組織的部分，這樣有利于對(duì)比。另一種稱為“外部對(duì)照”，有時(shí)在測(cè)試的切片中不存在已知的抗原，如在胃的標(biāo)本中懷疑是惡性黑色素瘤，需要用HMB45或Mart-1來(lái)檢測(cè)，在正常的胃組織中本身不存在相關(guān)的抗原，如果病變出現(xiàn)陽(yáng)性反應(yīng)結(jié)果，尚能提示是惡黑，但是如果出現(xiàn)陰性結(jié)果，就無(wú)法確定是本身組織中不含黑色素瘤抗原，還是技術(shù)問題。因此，應(yīng)另外設(shè)立一個(gè)已知的陽(yáng)性對(duì)照。這種在測(cè)試組織之外的陽(yáng)性對(duì)照稱為“外部對(duì)照”。在實(shí)際工作中需要設(shè)立外部對(duì)照的情況很多，如果每一種抗體都要選不同的陽(yáng)性對(duì)照，工作量會(huì)很大。為了解決這個(gè)問題，目前國(guó)內(nèi)外有單位將多種不同組織集成在一起，制成多組織切片、“臘腸”“春卷”切片、組織芯片等，其連續(xù)切片儲(chǔ)備待用，需要時(shí)取出一張便可作為陽(yáng)性對(duì)照。另外，比較簡(jiǎn)單的方法，是采用闌尾作為陽(yáng)性對(duì)照，因?yàn)榕c人體其它組織器官比較闌尾包含的組織種類較多，如有上皮、淋巴組織、平滑肌、間質(zhì)、神經(jīng)、間皮、血管等。一張闌尾切片可以檢測(cè)大多數(shù)常用的抗體。

To solve the problem of negative staining is very simple, is to set up a "positive control." If the positive control is expressed, indicating that the whole process of dyeing and all reagents are no problem. If the test piece is still negative at this time, it is a true negative, indicating that the tissue or cell does not have the corresponding antigen expression. Conversely, if the positive control is not colored, it indicates that there was a problem or some problem with the reagents during the dyeing process. One by one should find the reason. Positive controls include two, one called "self-control" or "internal control", which means that there are known antigens themselves in the test section, such as the presence of T and B cell antigens in normal lymph nodes, CD20 or CD3 All should be expressed. Self-control is an ideal control, the control and test tissues or cells are in the same slice, are in the same experimental conditions, the result is more reliable and more comparable. In the choice of their own photos of the best choice both the lesion and normal tissue part, which is conducive to the comparison. Another known as the "external control", and sometimes there is no known antigen in the test section, as in gastric specimens suspected of malignant melanoma, the need to use HMB45 or Mart-1 to detect, in normal gastric tissue Itself does not exist in the relevant antigen, if the lesions appear positive results, still can suggest evil, but if there is a negative result, it is not sure whether the tissue itself contains no melanoma antigen, or technical problems. Therefore, another known positive control should be set up. This positive control outside the test tissue is called the "external control." There are many situations where external controls need to be set up in practice. If each positive antibody is tested for a different positive control, the workload will be heavy. In order to solve this problem, at present, some domestic and foreign units integrate many different kinds of organizations to make multi-tissue slices, "sausage", "spring roll" slices, tissue chips and the like, and their serial slices are reserved for use. Can be used as a positive control. In addition, the relatively simple method is to use the appendix as a positive control, because compared with other tissues and organs of the human body appendix contains more tissue types, such as epithelial, lymphoid tissue, smooth muscle, interstitial, nerve, blood vessels, mesothelium and so on. An appendectomy can detect most commonly used antibodies.