肺炎克雷伯菌/銅綠假單胞菌聯(lián)合檢測試劑盒（PCR方法）

廣州健侖生物科技有限公司

One tube multiplex for detection AND quantification of Klebsiella pneumoniae, Pseudomonas aeruginosa and internal control.
單管多重檢測和定量肺炎克雷伯菌，銅綠假單胞菌和內(nèi)控。

肺炎克雷伯菌/銅綠假單胞菌聯(lián)合檢測試劑盒（PCR方法）

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（PCR方法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

7、細(xì)胞核著色
不適當(dāng)?shù)慕M織處理可以出現(xiàn)細(xì)胞核著色，如組織在二甲苯里浸泡時間太長（如從星期五浸泡到下星期一）、緩沖液中浸泡時間太長、組織變干、微波修復(fù)液的PH值和修復(fù)時間不當(dāng)或修復(fù)過程中修復(fù)液留下得太少，未沒過組織等。解決辦法是嚴(yán)格按照操作常規(guī)進行工作。
1、洗載玻片
將載玻片置于重鉻酸鉀和濃H2SO4混合液中，目的是為了是載玻片上的硅膠等除去，同時使一些肉眼看不見的凹凸不平的表面變平整，便于組織吸附，然后置于清水中清洗，除去殘余的重鉻酸鉀和濃H2SO4（大約沖一個小時左右），再將載玻片浸泡于酒精之中， 然后放到架子上，置于37 oC溫箱中，將多聚賴氨酸涂布于玻片的表面，由于Lys帶正電，而大多數(shù)的組織帶負(fù)電荷，從而產(chǎn)生吸附作用。
2、包埋組織
先在鐵模具中加入一些液態(tài)石蠟，先稍微冷卻，然后再將待包埋的組織置于石蠟之中，并排列整齊，再將塑料模具盒蓋上，zui后加入少許液體石蠟，進行冷凍，使石蠟變成固態(tài)。
3、切片
將包埋好的組織從模具上取下來，并置于石蠟切片機上，切片機通過調(diào)節(jié)上下左右來來使組織和切割方向*，然后調(diào)節(jié)切片的厚度，一般為5 um，如果比較難切，則可以適當(dāng)調(diào)整厚度，用毛筆將切割的載玻片向外拉，并用小鑷子將包含有完整組織的載玻片置于40 oC溫水中。

7, nuclear staining
Improper tissue handling can cause staining of the nucleus, such as tissue soaking in xylene for too long (eg soaking from Friday until next Monday), soaking in buffer for too long, tissue drying, and the pH of the microwave repair fluid and Repair time improper repair process or repair liquid left too little, have not been organized and so on. The solution is to strictly follow the routine work.
1, wash slides
Slides were placed in a mixture of potassium dichromate and concentrated H2SO4, the purpose is to remove the silica gel on the slide, etc., while some of the naked eye can see the uneven surface smooth, easy tissue adsorption, and then placed Clean water, remove the residual potassium dichromate and concentrated H2SO4 (about an hour or so), and then slides immersed in alcohol, and then placed on a shelf, placed in a 37 oC incubator, the poly Lysine is applied to the surface of glass slides. Since Lys is positively charged, most of the tissues are negatively charged, resulting in adsorption.
2, embedded tissue
First in the iron mold by adding some liquid paraffin, the first slightly cooled, then the tissue to be embedded in paraffin and arranged in neat rows, and then plastic mold cover, and finally add a small amount of liquid paraffin, frozen so that Paraffin becomes solid.
3, slice
The embedded tissue removed from the mold and placed on a paraffin slicer, slicer by adjusting the up and down to make the organization and cutting direction, and then adjust the thickness of the slice, usually 5 um, if more difficult to cut, The thickness can be adjusted appropriay, the cut slide is pulled outward with a brush and the slide containing the entire tissue is placed in 40 ° C warm water with a small forceps.