芬蘭流感B病毒診斷試劑盒

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芬蘭流感B病毒診斷試劑盒

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【公司名稱(chēng)】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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4、免疫組化中抗原修復(fù)的*條件是什么？（尤其是微波修復(fù)）
關(guān)于抗原修復(fù)，我試過(guò)的修復(fù)條件有EDTA熱修復(fù)，檸檬酸鈉為緩沖液的微波修復(fù)以及高壓鍋修復(fù)。從我的實(shí)驗(yàn)結(jié)果來(lái)看，微波修復(fù)其實(shí)很不容易掉片子的，而EDTA熱修復(fù)和高壓鍋修復(fù)是很容易掉片子的。因?yàn)榈羝又饕且驗(yàn)榧訜徇^(guò)程中產(chǎn)生的氣泡所引起，而微波修復(fù)水加熱到沸騰，沾在片子上的氣泡就會(huì)少，不會(huì)因?yàn)樾馀萆洗鹈撈?。做EDTA修復(fù)時(shí)，你可以將片子靠的盡量近些，或是在載玻片四周墊些棉花什么的，然后蓋上蓋玻片，這樣修復(fù)的話就能夠盡量減少載玻片上小氣泡的形成。我們用的微波修復(fù)條件為：2.94 g檸檬酸三鈉溶于1000 ml的水，調(diào)PH值至6.0，微波修復(fù)10分鐘。你可以試試，時(shí)間可以根據(jù)需要自己調(diào)整。對(duì)于檸檬酸修復(fù)來(lái)說(shuō)，只要高壓修復(fù)時(shí)間控制在加閥冒氣后90秒，冷水下終止開(kāi)高壓鍋蓋，高壓修復(fù)和微波修復(fù)一樣不掉片子，而且一般高壓修復(fù)效果往往更好一點(diǎn)；關(guān)于EDTA熱修復(fù)，一般說(shuō)明書(shū)上都講的是PH值等于9，實(shí)際上我試過(guò)，如果單傳用EDTA，很容易掉片子，但是用Tris-EDTA,一般就不會(huì)掉片子了，Tris-base的濃度為0.05 mmol/L,高壓和微波修復(fù)都可以，PH也等于9。

4, immunohistochemistry in the best conditions for antigen repair what? (Especially microwave repair)
On the antigen repair, I tried the repair conditions are EDTA thermal repair, sodium citrate buffer for microwave repair and pressure cooker repair. From my experimental results, microwave repair is actually very easy to remove the film, and EDTA thermal repair and pressure cooker repair is very easy to swap the film. Because the film is mainly caused by the bubbles generated during the heating process, and microwave repair water heated to boiling, the bubble on the film will be less, not because of small bubbles on the strings caused by the delamination. When doing EDTA repair, you can keep the film as close as possible, or pad some cotton around the slide, and then cover it with a coverslip, which will minimize the formation of small bubbles on the slide. We used the microwave repair conditions: 2.94 g trisodium citrate dissolved in 1000 ml of water, adjust the PH value to 6.0, microwave repair 10 minutes. You can try, time can be adjusted as needed. For the citric acid repair, as long as the high-pressure repair time control in the valve after venting 90 seconds, open the pressure cooker under cold water cover, high pressure repair and microwave repair can not afford the same film, and generally high-pressure repair is often a little better; about EDTA Heat repair, the general instructions are talking about the PH value is equal to 9, in fact, I tried, if the single-pass with EDTA, it is easy to lose the film, but with Tris-EDTA, generally will not drop the film, Tris-base concentration 0.05 mmol / L, high pressure and microwave repair can, PH is also equal to 9.