流感病毒A+B檢測(cè)試劑盒（免疫層析法）

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流感病毒A+B檢測(cè)試劑盒（免疫層析法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

5，背景強(qiáng)
1）考慮一抗?jié)舛雀撸?br />2）然后調(diào)整DAB孵育時(shí)間；
3）也要考慮血清封閉時(shí)間是否過(guò)短；
4）適當(dāng)增加抗體孵育后的浸洗次數(shù)和延長(zhǎng)浸洗時(shí)間等
石蠟制片法是將材料經(jīng)固定、脫水、透明、浸蠟后包埋在石蠟里面進(jìn)行切片的方法。此法適用范圍，制片手段完備，能將材料切成極薄的片子（可切至2-8微米左右），并能制成連續(xù)的片子，這是其它制片法難以達(dá)到的，因此在光學(xué)顯微鏡的制片技術(shù)上它是zui常用的一種方法。除了一些經(jīng)不住石蠟切片法中所應(yīng)用的各種藥劑處理的材料不能應(yīng)用石蠟切片以外，一般的材料都可以用此法制片，但有制作時(shí)間較長(zhǎng)，操作過(guò)程復(fù)雜以及材料易發(fā)硬或發(fā)脆等缺點(diǎn)。該法多采用旋轉(zhuǎn)式切片機(jī)進(jìn)行切片。
石蠟制片操作程序如下：
（一）材料的采集與分割
采集材料應(yīng)根據(jù)制片的目的和要求而定，材料要有代表性，無(wú)病蟲(chóng)害或其它損傷，如要觀察病理解剖，則要取病斑、病癥部位。采下的材料應(yīng)立即放在標(biāo)本箱或濕布包起來(lái)帶回實(shí)驗(yàn)室進(jìn)行分割固定（如有條件也可在試驗(yàn)地采集后進(jìn)行分割固定）。為使固定液能迅速的滲透材料，材料要進(jìn)行分割。分割時(shí)動(dòng)作要迅速，以防材料萎蔫。分割塊的大小，宜小不宜大，一般不超過(guò)1cm3，分割后立即殺死固定。

5, strong background
1) consider the high concentration of primary antibody;
2) Then adjust the DAB incubation time;
3) also consider whether the serum blocking time is too short;
4) appropriate to increase the number of antibody incubation after the dip and extend the immersion time
Paraffin preparation method is the material by the fixed, dehydrated, transparent, immersed wax embedded in paraffin inside the method of slicing. The scope of application of this method, the means of production is complete, the material can be cut into very thin films (can be cut to 2-8 microns), and can be made into a continuous film, which is difficult to achieve by other production methods, so in Light microscopy is technically the most commonly used method of making it. In addition to some paraffin sections can not be used in the treatment of various pharmaceutical agents can not use paraffin sections, the general material can be produced by this method, but with longer production time, the process is complicated and the material is easy to be hard or Crisp and other shortcomings. The law and more rotary slicing machine slicing.
Paraffin preparation procedures are as follows:
(A) material collection and segmentation
Collection materials should be based on the purpose and requirements of the film, the material should be representative, no pests or other damage, such as pathological anatomy to observe, you have to take lesions, the site of the disease. The collected material should be immediay put in the specimen box or wet cloth wrapped up and back to the laboratory for segmentation and fixation (if conditions can also be collected after the trial split and fixed). In order for the fixed liquid to rapidly penetrate the material, the material should be divided. Divide action to quickly, to prevent material wilting. Split the size of the block should be small should not be large, generally not more than 1cm3, killed immediay after the split.